KMID : 1024520090180121331
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Journal of the Environmental Sciences 2009 Volume.18 No. 12 p.1331 ~ p.1338
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Temporal Changes in Abundances of the Toxic Dinoflagellate Alexandrium minutum (Dinophyceae) in Chinhae Bay, Korea
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Park Tae-Gyu
Kang Yang-Sun
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Abstract
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Marine dinoflagellate Alexandrium minutum producing paralytic shellfish toxins is responsible for paralytic shellfish poisoning (PSP). To investigate its temporal distributions in Chinhae Bay where PSP occurs annually, SYBR Green¥° based A. minutum-specific real-time PCR probe was developed on the LSU rDNA region. Assay specificity and sensitivity were tested against related species, and its specificity was further confirmed by sequencing of field-derived samples. Ten months field survey in 2008 (a total 100 surface water samples) by using the real-time PCR probe showed that A. minutum was detected at very low densities of 1-4 cells L-1 in May and June being spring in Chinhae Bay, Korea.
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KEYWORD
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Alexandrium minutum, Dinoflagellate, PSP toxins, Real-time PCR
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